ABSTRACT
Objectives: To determine the prevalence of dry eye based on dry eye symptoms attending the Department of Ophthalmology and Medicine, Tripura Medical College. Materials and Methods: This study was conducted at Tripura Medical College, Hapania, Agartala, from 1st December 2009 to 31st December 2010. Complete history and ocular examinations was recorded on a pre designed proforma in the Department of Ophthalmology. The diagnosis was made from history and objective dry eye test in the following sequence: tear meniscus height, tear break up time test,fluorescein staining, schirmer test, and rose Bengal staining. If 2 or more of the above test were positive, the patient was deemed to be suffering from dry eye. All patients were sent to the Dept. of Medicine for systemic examination and to rule out any systemic diseases. Results: In this study, total number of patients that presented with dry eye symptoms were 762 and among them dry eye was diagnosed in 403 patients. The Prevalence rate was 3.10% and it was highest in the age of more than 70 years of age. Females had higher prevalence (1.94%) than males (1.15%). Conclusion: The prevalence of dry eye is less in Tripura at around 3.10%. This may be due to the geographical and climatic implication with a high degree of humidity reigning in this region.
Subject(s)
Adult , Aged , Coloring Agents/diagnosis , Diagnostic Techniques, Ophthalmological/methods , Dry Eye Syndromes/diagnosis , Dry Eye Syndromes/epidemiology , Dry Eye Syndromes/etiology , Female , Fluorescein/diagnosis , Hospitals , Humans , Male , India , Population , Prevalence , Rose Bengal/diagnosis , Staining and Labeling/methods , Tears/metabolismABSTRACT
In the present study, isolation of anaerobic bacteria from 24 different eco-niches was carried out. A total number of 300 bacterial isolates, including 230 obligate and 70 facultative anaerobes were obtained using anaerobic techniques. All the isolates were initially screened for succinic acid production by Fluorescein test and TLC method. During screening, 10 isolates found to produce succinic acid were further examined by HPLC and then finally confirmed for succinic acid by LC-MS analysis. Amongst 10 isolates, isolate SAP, a facultative anaerobe isolated from buffalo rumen fluid, showed maximum yield of 2.1 g/l of succinic acid from 10 g of glucose in 24 hr under anaerobic condition. This isolate was identified as Klebsiella pneumoniae strain SAP by 16S rDNA sequence and signature sequence analysis. Mouse lethality test for the strain SAP showed LD50 value of 3.3 x 10(8) CFU/ml, which shows non-virulent nature of the strain. This strain may become a candidate strain for succinic acid production because of its osmotolerant nature and higher succinate:acetate ratio.
Subject(s)
Acetates/metabolism , Animals , Bacteria, Anaerobic/metabolism , Bacterial Proteins/metabolism , Bacterial Toxins/analysis , Base Sequence , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Fluorescein/diagnosis , Gas Chromatography-Mass Spectrometry , Humans , Klebsiella pneumoniae/classification , Mice , Osmosis/physiology , RNA, Ribosomal, 16S/genetics , Succinic Acid/metabolism , VirulenceABSTRACT
Nasolacrimal duct injury is a well established complication of functional endoscopic sinus surgery. In 1992, Bolger reported an incidence of nasolacrimal duct injuries in endoscopic sinus surgery of up to 15 per cent, but there is no documentation in microscopic sinus surgery. Fluorescein instillation into the lacrimal system via the punctum was done to determine the incidence of nasolacrimal duct injuries in 16 patients who underwent 32 microscopic sinus procedures. Only one patient exhibited nasolacrimal duct injury intraoperatively on the left side (0.3%). He had complete healing of the nasolacrimal duct at 2 months and no postoperative epiphora developed.